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An integrated single cell and spatial transcriptomic map of human white adipose tissue.

Department of Medicine Huddinge (H7), Karolinska Institutet, Karolinska University Hospital Huddinge, SE-141 83, Huddinge, Sweden. Laboratory of Translational Nutrition Biology, Institute of Food, Nutrition and Health, Department of Health Sciences and Technology, ETH Zurich, Schwerzenbach, Switzerland. Helmholtz Institute for Metabolic, Obesity and Vascular Research (HI-MAG) of the Helmholtz Zentrum München at the University of Leipzig and University Hospital Leipzig, Leipzig, Germany. Center for Infectious Medicine, Department of Medicine Huddinge (H7), Karolinska Institutet, Karolinska University Hospital Huddinge, SE-141 52, Huddinge, Sweden. Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, SE-171 77, Stockholm, Sweden. Department of Clinical Science, Intervention & Technology (CLINTEC), Unit of Renal Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, SE-141 86, Huddinge, Sweden. Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, SE-171 65, Solna, Sweden. Department of Metabolic Health, Nestle Institute of Health Sciences, Nestle Research, Lausanne, Switzerland. Department of Obesity and Nutritional Sciences, The Novo Nordisk Foundation, Hellerup, Denmark. Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre(+), Maastricht, the Netherlands. Institute of Metabolic and Cardiovascular Diseases (I2MC), Institut National de la Santé et de la Recherche Médicale (Inserm), Université Toulouse III - Paul Sabatier (UPS), Université de Toulouse, UMR1297, Toulouse, France. Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Charles University, Prague and Université Toulouse III - Paul Sabatier (UPS), Toulouse, France. Laboratoire de biochimie, Centre Hospitalier Universitaire de Toulouse, Toulouse, France. Institut Universitaire de France (IUF), Paris, France. Medical Department III - Endocrinology, Nephrology, Rheumatology, University of Leipzig Medical Center, Leipzig, Germany. Department of Medicine Huddinge (H7), Karolinska Institutet, Karolinska University Hospital Huddinge, SE-141 83, Huddinge, Sweden. mikael.ryden@ki.se. Department of Medicine Huddinge (H7), Karolinska Institutet, Karolinska University Hospital Huddinge, SE-141 83, Huddinge, Sweden. niklas.mejhert@ki.se.

Nature communications. 2023;(1):1438
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Abstract

To date, single-cell studies of human white adipose tissue (WAT) have been based on small cohort sizes and no cellular consensus nomenclature exists. Herein, we performed a comprehensive meta-analysis of publicly available and newly generated single-cell, single-nucleus, and spatial transcriptomic results from human subcutaneous, omental, and perivascular WAT. Our high-resolution map is built on data from ten studies and allowed us to robustly identify >60 subpopulations of adipocytes, fibroblast and adipogenic progenitors, vascular, and immune cells. Using these results, we deconvolved spatial and bulk transcriptomic data from nine additional cohorts to provide spatial and clinical dimensions to the map. This identified cell-cell interactions as well as relationships between specific cell subtypes and insulin resistance, dyslipidemia, adipocyte volume, and lipolysis upon long-term weight changes. Altogether, our meta-map provides a rich resource defining the cellular and microarchitectural landscape of human WAT and describes the associations between specific cell types and metabolic states.

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Publication Type : Meta-Analysis

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